ID: 1016 Production of endothelial progenitor cells from skin fibroblasts by direct reprogramming for clinical usages

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Direct Reprogramming of Murine Fibroblasts to Hematopoietic Progenitor Cells

Recent reports have shown that somatic cells, under appropriate culture conditions, could be directly reprogrammed to cardiac, hepatic, or neuronal phenotype by lineage-specific transcription factors. In this study, we demonstrate that both embryonic and adult somatic fibroblasts can be efficiently reprogrammed to clonal multilineage hematopoietic progenitors by the ectopic expression of the tr...

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Direct Conversion of Adult Skin Fibroblasts to Endothelial Cells by Defined Factors Running title :

Background—Cell-based therapies to augment endothelial cells (ECs) hold great therapeutic

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Direct conversion of adult skin fibroblasts to endothelial cells by defined factors.

BACKGROUND Cell-based therapies to augment endothelial cells (ECs) hold great therapeutic promise. Here, we report a novel approach to generate functional ECs directly from adult fibroblasts. METHODS AND RESULTS Eleven candidate genes that are key regulators of endothelial development were selected. Green fluorescent protein (GFP)-negative skin fibroblasts were prepared from Tie2-GFP mice and...

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Direct reprogramming of fibroblasts into endothelial cells capable of angiogenesis and reendothelialization in tissue-engineered vessels.

The generation of induced pluripotent stem (iPS) cells is an important tool for regenerative medicine. However, the main restriction is the risk of tumor development. In this study we found that during the early stages of somatic cell reprogramming toward a pluripotent state, specific gene expression patterns are altered. Therefore, we developed a method to generate partial-iPS (PiPS) cells by ...

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Direct Reprogramming of Human Dermal Fibroblasts Into Endothelial Cells Using ER71/ETV2.

RATIONALE Direct conversion or reprogramming of human postnatal cells into endothelial cells (ECs), bypassing stem or progenitor cell status, is crucial for regenerative medicine, cell therapy, and pathophysiological investigation but has remained largely unexplored. OBJECTIVE We sought to directly reprogram human postnatal dermal fibroblasts to ECs with vasculogenic and endothelial transcrip...

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ژورنال

عنوان ژورنال: Biomedical Research and Therapy

سال: 2017

ISSN: 2198-4093,2198-4093

DOI: 10.15419/bmrat.v4is.294